Direct photoreactive immobilization of water-soluble phospholipid polymers on substrates in an aqueous environment

The purpose of this study is to achieve a simpler and safer surface modification substrates using photoreactive polymer in an aqueous environment. We synthesized water-soluble polymers with both phenylazide groups phosphorylcholine groups, poly(2-methacryloyloxyethyl phosphorylcholine-co-4-methacryl tetra(ethylene glycol)oxycarbonyl-4-phenylazide) (PMEPAz), via reversible addition fragmentation chain transfer polymerization. PMEPAz different polymerization degrees were well-defined structure. To immobilize on the substrate by photoreaction, it necessary adsorb solution because chemically bind hydrogen abstract reaction. relationship between solubilization state adsorption behavior at was investigated. began form unstable molecular aggregates concentration 10?2 mg/mL formed stable 100 mg/mL. At 10?1 mg/mL, solution, resulting maximization amount adsorbed effective photoreaction substrate. thickness reacted layer increased increase degree, uniform 3.4 nm when degree 400. After modification, hydrophobic surfaces original became hydrophilic. Additionally, fibrinogen platelet adhesion effectively suppressed based characteristics unit.